We report a study that developed an edta disk screen test and a molecular diagnostic assay for the detection of mblproducing pseudomonas aeruginosa. Endemic carbapenemresistant pseudomonas aeruginosa with. Several types of mbl enzymes have been identified in p. Multidrugresistant pseudomonas aeruginosa producing per1. Extended spectrum betalactamase and metallo betalactamase. Combination therapy including arbekacin abk, an aminoglycoside antibiotic, has.
Novel bacteriophage therapy for controlling metallo betalactamase producing pseudomonas aeruginosa infection in catfish. There have been few clinical studies to define the optimal therapy for infections caused by esbl producing pseudomonas aeruginosa strains. The production of mbl in clinical isolates of pseudomonas. Although mbls were first detected in spain in 2002, very few mbl producing p. Limited data is available on irmblppa carriers and their role as source andor reservoir of nosocomial infection. Epidemiology and virulence of vim4 metallobetalactamase. Karadesai, prevalence of extendedspectrum betalactamases, ampc betalactamase, and metallobetalactamase producing pseudomonas aeruginosa and acinetobacter baumannii in an intensive care unit in a tertiary care hospital, journal of the scientific society, vol.
Infection with mbl producing strains represents a therapeutic problem due to their resistance to all beta lactams except monobactams. Imipenemresistant metallo beta lactamase positive pseudomonas aeruginosa irmblppa infections occur as outbreaks and epidemics with a potential to spread within and between hospitals and intercontinentally. Pseudomonas species from cattle dung producing extended. Frequently encountered multidrugresistant bacterial isolates of p. Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. Prevalence and clonal dissemination of metallobetalactamase. Emergence of vim2 metallo beta lactamase producing pseudomonas aeruginosa isolates in a paediatric hospital in serbia. Pdf burn patients infected with metallobetalactamase. During september 18november 19, 2018, cdc received 31. Pseudomonas aeruginosa with acquired metallo beta lactamase determinants in a european hospital. Metallo beta lactamase mbl is an emerging resistance mechanism among pseudomonas aeruginosa. Briefly, thirty confirmed isolates were selected to be tested for their mbl enzyme production by e test. This study was to determine the extended spectrum beta lactamase esbl and metallo beta lactamase mbl production in pseudomonas species from cattle fecal samples.
In this work we aimed at studying the occurrence of metallo beta lactamase mbl producing p. So the main purpose of our study was to determine the burden of esbl and mbl production in e. Emergence and dissemination of multidrug resistant clones. Molecular detection of metallo beta lactamase producing pseudomonas aeruginosa isolated from different sites of infection. Metallobetalactamase producing pseudomonas aeruginosa in a healthcare setting in alexandria, egypt. These antibiotics all have a common element in their. Emergence of carbapenemhydrolysing metallo beta lactamase mbl in pseudomonas aeruginosa has been described worldwide. The metallo beta lactamase mbl producing bacterial isolate was evaluated using imipenem ethylenediaminetetraacetic acid edta disk method.
This study aimed to characterize a comprehensive collection of mblproducing p. Pdf since about twenty years, following the introduction into therapeutic of news betalactam antibiotics broadspectrum cephalosporins, monobactams. In the present study, we describe the first nosocomial outbreak of infection caused by blavim2positive p. This study aimed to characterize a comprehensive collection of mbl producing p. Carbapenemresistant isolates of pseudomonas aeruginosa producing metallo. Metallo beta lactamase producing pseudomonas aeruginosa and. The aim of this study was to detect mbl producing p. From this it can be inferred that metallo beta lactamases producing pseudomonas aeruginosa are some what lower in incidence in our narayana general hospital. In 2001, multidrugresistant pseudomonas aeruginosa carrying blavim mbl genes were found to be widespread approximately 20% of all p. This study was designed to explore the dissemination of pseudomonas aeruginosa producing esbls on their plasmids recovered from the. In pseudomonas aeruginosa, secondary beta lactamases with extended substrate specificity can be responsible for acquired resistance to the most powerful antipseudomonal beta lactams, such as expandedspectrum cephalosporins and carbapenems.
Journal of pathogens hindawi publishing corporation. Role of imipenemresistant metallobetalactamase positive. We aimed to describe the prevalence and molecular characteristics of spm1like, imp1like and vim type mbls among ceftazidime andor imipenemresistant nosocomial p. Nosocomial spread of pseudomonas aeruginosa producing the. Molecular detection of metallobeta lactamase producing. Inducible amp c betalactamase producing pseudomonas. Arbekacin treatment of a patient infected with a pseudomonas. Metallobetalactamase producing pseudomonas aeruginosa in. Extended spectrum and metalo betalactamase producing.
Extended spectrum beta lactamase esbl and metallo beta lactamase mbl production in klebsiella pneumoniae and escherichia coli are the commonest modes of drug resistance among these commonly isolated bacteria from clinical specimens. Indiscriminate use of antibiotics in livestock contributes to emergence of antimicrobial resistance in pathogens cohabiting the gastrointestinal tract of animals. Spread of vim2 metallobetalactamase in pseudomonas. The aim of this study was to investigate the molecular epidemiological characteristics of metallo. We first assessed the 28day inhospital allcause mortality in patients with verona integronencoded metallo.
Phenotypic detection of carbapenem resistance among pseudomonas aeruginosa isolated from patients with diabetic foot infections. Our study aimed to investigate the effect of mblproducing p. Pseudomonas aeruginosa, epsilometer test, metallo beta lactamases, mbl encoding genes. Evaluation of phenotypic tests for detection of metallo. The bacteriophage therapy is an effective antimicrobial approach with potentially important applications in medicine and biotechnology which can be seen as an additional string in the bow.
Studies on various gramnegative bacteria suggest that resistance to carbapenem antibiotics is responsible for increased mortality in patients. Mortality associated with carbapenemsusceptible and verona. Pseudomonas aeruginosa is one of the most important causes of nosocomial infections, and its eradication is very difficult due to its multidrug resistance. Metallobetalactamase producing pseudomonas aeruginosa in a. The majority of patients were medical tourists who had weight loss surgery at a single facility in tijuana, mexico. Metallo beta lactamase producing pseudomonas aeruginosa. Among the isolates nonsusceptible to imipenem that were collected from 28 hospitals from 2000 to 2001, 44 11. The causative organism was a strain of pseudomonas putida that produced a metallo beta lactamase.
During september 18november 19, 2018, cdc received 31 reports of vimcrpa. Oct 23, 2017 frequently encountered multidrugresistant bacterial isolates of p. Pdf pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a. Docquier j, luzzaro f, amicosante g, toniolo a, rossolini g. Prevalence of extendedspectrum beta lactamases, ampc beta lactamase and metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii. Karadesai, prevalence of extendedspectrum beta lactamases, ampc beta lactamase, and metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii in an intensive care unit in a tertiary care hospital, journal of. This study was carried out to determine the prevalence of metallo. Episodes of ventilatorassociated pneumonia were recorded on days 5 and 12. Emerging drug resistant bacteria in aquaculture industry due to unrestricted use of antibiotics warrants more sustainable and environmental friendly strategies for controlling fish infections. An evaluation of four different phenotypic techniques for detection of. Oct 23, 20 the patient regained consciousness on day 8 postadmission. Dissemination of pseudomonas aeruginosa producing spm1. Mortality associated with carbapenemsusceptible and.
A prospective study was performed over a period of 15 month in a tertiary care hospital. Epidemiology and virulence of vim4 metallo beta lactamase producing pseudomonas aeruginosa isolated from burn patients in eastern algeria. Multidrugresistant pseudomonas aeruginosa producing per1 extendedspectrum serine. Detection of pseudomonas aeruginosa producing metallo beta lactamases in a large centralized laboratory. So, the main aims of this study were to determine the rates of biofilm production and metallo beta lactamase production mbl among the strains of pseudomonas aeruginosa. Risk factors for nosocomial infections due to pseudomonas aeruginosa producing metallo beta lactamase in two tertiarycare teaching hospitals j antimicrob chemother, 58 2006, pp. Metallo beta lactamase mbl producing pseudomonas aeruginosa in the burn patients is a leading cause of morbidity and mortality and remains a serious health concern among the clinicians. Jul 16, 2008 cardoso o, alves af, leitao r 2008 metallo. The objective of the present study was to characterize the metallo beta lactamases mbls, integrons, oprd modifications and virulence factors of p. First identification of pseudomonas aeruginosa isolates producing a kpctype carbapenemhydrolyzing betalactamase. One of the most important resistant mechanisms in gramnegative bacteria is extended spectrum. The resistance by these organisms are in a rise against all antibiotics. Pseudomonas aeruginosa is well adapted to the hospital setting and can cause a wide array of nosocomial infections that occasionally culminate in recalcitrant outbreaks. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii s john, balagurunathan r abstract this study aims in identifying mbls particularly zn requiring molecular class b enzymes produced by pseudomonas aeruginosa and acinetobacter baumannii.
Increased mortality rates have been documented for patients infected with mbl producing pseudomonas aeruginosa, and these rates are especially due to. Epidemiology and virulence of vim4 metallo beta lactamase producing pseudomonas aeruginosa isolated from burn patients in eastern algeria author links open overlay panel samah meradji a abouddihaj barguigua b c mohamed cherif bentakouk d kaotar nayme b c khalid zerouali c dekhil mazouz e houria chettibi a mohammed timinouni b. In future this scenario may change because the gene responsible for mbl production is carried on mobile genetic element resulting in rapid spread to sensitive bacteria in the hospital. The bla vim2 gene was detected in 75% isolates and bla. Dominance of international highrisk clones among metallo. Combination therapy with arbekacin and levofloxacin successfully resolved the pneumonia. The aim of this study was to determine the prevalence and pattern of antibiotic resistance, extended spectrum and metallo beta lactamase producing p. Beta lactamase enzymatic activity can be detected using nitrocefin, a chromogenic cephalosporin substrate which changes color from yellow to red upon beta lactamase mediated hydrolysis.
Infections caused by pseudomonas putida strains that produce metallo beta lactamases mbls are difficult to treat because effective antibiotics are lacking. A prospective study was performed over a period of 8 months in a tertiary care hospital. Predominant resistance mechanism and a threat in a tertiary care teaching hospital nandini mp1, navaneeth bv2 1assistant surgeon, sri jayadeva institute of cardiovascular sciences and research, bangalore, karnataka. Emergence of metallo betalactamase producing pseudomonas aeruginosa in pakistan. Epidemiology and characteristics of metallo lactamase. Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both develop ing and developed countries. Detection of pseudomonas aeruginosa producing metallo. Verona integronencoded metallobetalactamaseproducing. Metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter baumannii. Biofilm and metallo betalactamase production among the. During 20182019, cdc identified a multistate outbreak of a highly resistant strain of pseudomonas aeruginosa verona integronencoded metallo. Beta lactamase provides antibiotic resistance by breaking the antibiotics structure. Pseudomonas aeruginosa is one of the most common causes of nosocomial infections. Prevalence of extendedspectrum betalactamases, ampc beta.
Characterization by phenotypic and genotypic methods of metallo. A number of these enzymes have been described, including extendedspectrum serine beta lactamases esbls of groups 2be and. Metallobetalactamase producing pseudomonas aeruginosa. The appearance of metallo beta lactamase mbl producing pseudomonas aeruginosa and acinetobacter sp. So, the main aims of this study were to determine the rates of biofilm production and metallo betalactamase production mbl among the strains of pseudomonas aeruginosa and acinetobacter spp. Harbourrelated genes on plasmids, increase the risk of resistance transmission among commonly reported hospital infections. Pdf molecular epidemiology and clinical characteristics. Metallo beta lactamase mbl producing pseudomonas aeruginosa in the burn patients is a leading cause of morbidity and mortality and remains a serious health concern among the. Four types of mbls, imp, vim, spm1, and gim1, have been reported in pseudomonas aeruginosa, which is a leading cause of nosocomial infections 3, 11, 29. Blood stream infections bsi with pseudomonas aeruginosa lead to poor clinical outcomes. Metallo beta lactamase producing pseudomonas aeruginosa in a healthcare setting in alexandria, egypt. This study was undertaken to detect esbl, ampc beta lactamase and metallo beta lactamase producing pseudomonas aeruginosa and acinetobacter species from the endotracheal aspirates. Sep 19, 2017 extended spectrum beta lactamase esbl and metallo beta lactamase mbl production in klebsiella pneumoniae and escherichia coli are the commonest modes of drug resistance among these commonly isolated bacteria from clinical specimens.
Jovcic b, vasiljevcic z, djukic s, topisirovic l, kojic m. Pdf metallobetalactamaseproducing pseudomonas aeruginosa. Pseudomonas aeruginosa is a common nosocomial pathogen, particularly among immunocompromised patients. Lee k, lim jb, yum jh, yong d, chong y, kim jm, bla vim2 cassettecontaining novel integrons in metallo. The bacteriophage therapy is an effective antimicrobial approach with potentially important. Prevalence of metallolactamases producing pseudomonas. Metallo beta lactamase production is an important mechanism for carbapenem resistance of pseudomonas aeruginosawhich, represents an emerging public health challenge. The prevalence of this mechanism is particularly high in latin america. Colistin, the traditional drug of last resort, is not available in japan, and the choice of antibiotic treatment for p. High prevalence of imipenemresistant and metallobeta. Lactamase producing carbapenemresistant pseudomonas aeruginosa or vimcrpa. Novel bacteriophage therapy for controlling metallobeta. Biofilm and metallo betalactamase production among the strains of. Nosocomial infections with metallobetalactamaseproducing.
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